Infrastructure & services

The current infrastructure used within the PerMed: T2BA involves several unique technologies and research services across the partner institutions. The research infrastructure was built-up within the large research infrastructure projects (e.g EATRIS-CZ, BBMRI-CZ, CZ-OPENSCREEN, ELIXIR-CZ, NCMG and CZ-BIOIMAGING, or developed by private partners:

Genomics

• HTS qPCR in 1536 format, hybridization platform, NGS, mass spectrometry based DNA analysis

Proteomics

• MALDI-TOF/TOFs, HPLC-MS, qTRAP, qTOF, Orbitrap mass spectrometers

Metabolomics

• GC-TOF, qTRAP, Orbitrap mass spectrometers

Microscopy

• AFM, Raman microscopy, IR microscopy, confocal spinning disc and laser scanning microscopy, superresolution, PALM, SIM, TIRF, transmission and raster EM)
  HTS/HCA analysis (compound collection+dispensing, 3-arm robotic system for screening of small molecules in BSL2+/BSL3 and/or hypoxic environment, irradiation, readers: fluorescence, luminesence, radioactivity, absorbance, wide field confocal HCA, mass spectrometry based screening (RapidFire plus qTRAP)

BSL3 laboratories

• Research with major human pathogens

Small animal imaging center

• Optical (fluorescence, luminescence), X-ray, PET/SPECT/CT, ultrasound, access to MRI

Animal facilities and models

• For cancer, genetic, infection, neuro and cardio research using small
  and large animals, GLP certified animal studies (toxicology)

Radiochemistry, medicinal, organic, macromolecular, nanoparticle and combinatorial chemistry

Biobank

• >35.000 highly characterized tissue/blood/plasma/bone marrow samples

Cyclotron

With radionuclide production, purification and characterization of tracers for SPECT/PET imaging

Early clinical trials unit

GMP facilities

• For manufacturing of ATMPs (contractual)

Certified clinical diagnostics and testing laboratories

• ISO 17025 and ISO 15189

Scale-up laboratories

• For synthesis of (pre)clinical batches of small molecules